PI简介赖 仞 研究员　博士生导师

1972年7月出生于四川省资中县。2001年8月在中国科学院昆明动物研究所获得博士学位。2002－2004年在英国利物浦大学和美国伯明韩阿拉巴马大学从事博士后研究。目前，正主持多项国家基金、中科院重点项目和云南省重点基金项目。2005年获得中国科学院“百人计划”择优支持。

研究方向

a、发掘了大量的抗菌肽，并对其结构、功能和基因进行了全面的研究。目前我们已从动物、微生物中发现了300种以上的抗菌肽，属于65个不同的多肽家族，占目前世界已知抗菌肽总数1500种（110个家族）的20％以上。代表性成果发表于Molecular cellular proteomics (2007), Molecular immunology (2008), Eur J Immunol (2006).

b、设计并改造了抗菌肽，使之同时具有抗菌和抑制胰蛋白酶水解活性；也改造了抗菌肽使之在胃酸环境发挥强抗菌活性。这些研究结果主要发表于FASEB J （2007）和FASEB J （2004）。这些改造的双功能多肽为抗菌肽的体内应用打下了坚实基础。

c、全面研究了吸血节肢动物（蜱和牛虻）与其宿主相互作用的分子机制，揭示了牛虻作为传统抗血栓药物的物质基础。这些研究结果主要发表于Molecular cellular proteomics (2008)。

d、发掘了大量的可以分泌具有应用前景活性多肽与蛋白的微生物资源，并进行了物种鉴定。发现了10多个微生物新种，5个新属，1个新科(中华杆菌科Sinobacteraceae)。中华杆菌科也是目前我国唯一自己发现命名的格兰氏阴性细菌新科。代表性结果分别发表于Int J Syst Evol Microbiol （2006，2007，2008）。

e、发现了新型皮肤抗氧化系统，并对两栖动物皮肤抗氧化多肽组学进行研究。代表性结果分别发表于Mol Cell Proteomics. 2008，Nov 20. [Epub ahead of print]

f、在这些工作基础上，发现了大量具有临床应用前景的活性多肽与蛋白，建立了相应的生物活性分子资源库，申请了35个发明专利，其中的15个已经获得授权

科研任务（2003-今）

注：A为承担的项目总数，B为主持的项目数。

研究团队（2003-今）

发表论文

2008年发表论文

[1]Yang H, Wang X, Liu X, Wu J, Liu C, Gong W, Zhao Z, Hong J, Lin D, Wang Y, Lai R*: Antioxidant peptideomics reveals novel skin antioxidant system. Mol Cell Proteomics 2008, Nov 20. [Epub ahead of print]

[2]Xu X, Yang H, Ma D, Wu J, Wang Y, Song Y, Wang X, Lu Y, Yang J, Lai R*: Toward an understanding of the molecular mechanism for successfully blood-feeding by proteomics analysis coupling with pharmacological testing of horse fly salivary glands. Mol Cell Proteomics 2008, 7: 582-590.

[3]Wang Y, Hong J, Liu X, Yang H, Liu R, Wu J, Wang A, Lin D, Lai R*: Snake cathelicidin from Bungarus fasciatus is a potent peptide antibiotics. PLoS ONE 2008, 3: e3217.

[4] Li J, Wu H, Hong J, Xu X, Yang H, Wu B, Wang Y, Zhu J, Lai R*, Jiang X, Lin D, Prescott MC, Rees HH: Odorranalectin is a small peptide lectin with potential for drug delivery and targeting. PLoS ONE 2008, 3: e2381.

[5]Yan X, Feng H, Yu H, Yang X, Liu J, Lai R*: An immunoregulatory peptide from salivary glands of the horsefly, Hybomitra atriperoides. Dev Comp Immunol 2008, 32: 1242-1247.

[6]Lu Y, Ma Y, Wang X, Liang J, Zhang C, Zhang K, Lin G, Lai R*: The first antimicrobial peptide from sea amphibian. Mol Immunol 2008, 45: 678-681.

[7]Zhou Y, Zhang YQ, Zhi XY, Wang X, Dong J, Chen Y, Lai R*, Li WJ*: Description of Sinobacter flavus gen. nov., sp. nov., and proposal of Sinobacteraceae fam. nov. Int J Syst Evol Microbiol 2008, 58:184-189.

[8]Liu SB, He YY, Zhang Y, Lee WH, Qian JQ, Lai R, Jin Y: Crystallin and trefoilgbY, A non-lens factor complex from amphibian skin and its functional implications. PLoS ONE 2008, 3: e1770.
[9]Wang AL, Wang JY, Feng H, Yang HL, Lai R: A novel family of antimicrobial peptides from the skin of Amolops loloensis. Biochimie 2008, 90: 863-867.
[10]Lu X, Ma Y, Wu J, Lai R: Two serine protease inhibitors from the skin secretions of the toad, Bombina microdeladigitora. Comp Biochem Physiol B 2008, 149: 608-612.

[11]Lu J#, Yang H, Yu H, Gao W, Lai R*, Liu J*, Liang X: A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides 2008, 29: 369-374.

[12]Yang H#, Xu X, Ma D, Zhang K, Lai R*: A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith). Toxicon 2008, 51: 289-296.

[13]Liu X, You D, Chen L, Wang X, Zhang K, Lai R*: A novel bradykinin-like peptide from skin secretions of the frog, Rana nigrovittata. J Pept Sci 2008, 14: 626-630.

[14]Han Y, Yu H, Yang X, Rees HH, Liu J, Lai R*: A serine proteinase inhibitor from frog eggs with bacteriostatic activity. Comp Biochem Physiol B 2008,149: 58-62.

[15]Che QL, Zhou Y, Yang HL, Li JX, Lai R*: A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami. Peptides 2008, 29: 529-535.

[16]Han J, Yang H, Xu X, Wu J, Wang Y, Meng Q, Lai R*: An anticoagulant serine protease from the wasp venom of Vespa magnifica (Smith). Toxicon 2008, 51: 914-922.

[17]Liu H, Liu R, Yang SY, Gao WK, Zhang CX, Zhang KY, Lai R*: Flavobacterium anhuiense sp. nov., isolated from field soil. Int J Syst Evol Microbiol 2008, 58: 756-760.

[18]Wei W, Zhou Y, Wang X, Huang X, Lai R*: Sphingobacterium anhuiense sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2008, 58: 2098-2101.

[19]Liu R, Liu H, Yang S, Zhang K, Lai R*: Paenibacillus kuerleensis sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 2008 (IJSEM 65633).

[20]Liu R, Liu H, Zhang CX, Yang SY, Liu XH, Zhang KY, Lai R*: Sphingobacterium siyangense sp. nov., isolated from farm soil. Int J Syst Evol Microbiol 2008, 58: 1458-1462.

[21]Liu R, Liu H, Feng H, Wang X, Zhang CX, Zhang KY, Lai R*: Pseudomonas duriflava sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 2008, 58: 1404-1408.

[22]Zhang C, Liu J, Xu M, Sun J, Yang S, An X, Gao G, Lin M, Lai R, He Z, Wu Y, Zhang K: Heterorhabditidoides chongmingensis gen. nov., sp. nov. (Rhabditida: Rhabditidae), a novel member of the entomopathogenic nematodes. J Invertebr Pathol 2008, 98: 153-168.

[23]Zhou Y, Wei W, Che Q, Xu Y, Wang X, Huang X, Lai R*: Bacillus pallidus sp. nov., isolated from forest soil. Int J Syst Evol Microbiol 2008, 58(Pt 12): 2850-2854.

2007年发表论文

[1]Li J, Xu X, Xu C, Zhou W, Zhang K, Yu H, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J: Anti-infection peptidomics of amphibian skin. Mol Cell Proteomics 2007, 6: 882-894.

[2]Li J, Zhang C, Xu X, Wang J, Yu H, Lai R*, Gong W*: Trypsin inhibitory loop is an excellent lead structure to design serine protease inhibitors and antimicrobial peptides. FASEB J 2007, 21: 2466-2473.

[3]Li J, Yu H, Xu X, Wang X, Liu D, Lai R*: Multiple bombesin-like peptides with opposite functions from skin of Odorrana grahami. Genomics 2007, 89: 413-418.

[4]Wang X, Song Y, Li J, Liu H, Xu X, Lai R*, Zhang K: A new family of antimicrobial peptides from skin secretions of Rana pleuraden. Peptides 2007, 28: 2069-2074.

[5]Yu H, Wang X, Liu J, Lai R*: A novel algesic peptide derived from skin secretions of the frog Amolops loloensis. J Pept Sci 2007, 13: 798-802.

[6]Chen L, Li Y, Li J, Xu X, Lai R*, Zou Q*: An antimicrobial peptide with antimicrobial activity against Helicobacter pylori. Peptides 2007, 28: 1527-1531.

[7]Liu X, Wang Y, Cheng L, Song Y,Lai R*: Isolation and cDNA cloning of cholecystokinin from the skin of Rana nigrovittata. Peptides 2007, 28: 1540-1544.

[8]Zhou Y, Dong J, Wang X, Huang X, Zhang KY, Zhang YQ, Guo YF, Lai R*, Li WJ*: Chryseobacterium flavum sp. nov., isolated from polluted soil. Int J Syst Evol Microbiol 2007, 57: 1765-1769.

[9]Yu H, Yang H, Ma D, Lv Y, Liu T, Zhang K, Lai R*, Liu J: Vespid chemotactic peptide precursor from the wasp, Vespa magnifica (Smith). Toxicon 2007, 50: 377-382.

[10]Xu C, Ma D, Yu H, Li Z, Liang J, Lin G, Zhang Y, Lai R*: A bactericidal homodimeric phospholipases A2 from Bungarus fasciatus venom. Peptides 2007, 28: 969-973.

[11]Zhou Y, Wang X, Liu H, Zhang KY, Zhang YQ, Lai R*, Li WJ*: Pontibacter akesuensis sp. nov., isolated from a desert soil in China. Int J Syst Evol Microbiol 2007, 57: 321-325.

2006年发表论文

[1]Xu X, Yang H, Yu H, Li J, Lai R*: The mastoparanogen from wasp. Peptides2006, 27: 3053-3057.

[2]Li J, Liu T, Xu X, Wang X, Wu M, Yang H, Lai R*: Amphibian tachykinin precursor. Biochem Biophys Res Commun 2006, 350: 983-386.

[3]Lu Y, Li J, Yu H, Xu X, Liang J, Tian Y, Ma D, Lin G, Huang G, Lai R*: Two families of antimicrobial peptides with multiple functions from skin of rufous-spotted torrent frog, Amolops loloensis. Peptides 2006, 27: 3085-3091.

[4]Zhou Z, Yang H, Xu X, Wang X, Lai R*: The first report of kininogen from invertebrates. Biochem Biophys Res Commun 2006, 347: 1099-1102.

[5]Liang J, Han Y, Li J, Xu X, Rees HH, Lai R*: A novel bradykinin-like peptide from skin secretions of rufous-spotted torrent frog, Amolops loloensis. Peptides 2006, 27: 2683-2687.

[6]李建许，刘彤光，杨海龙，徐学清，刘志刚，赖仞*：从中华硬蜱神经节中分离出两种神经肽。科学通报 2006, 51: 2501-2504.

[7]Yu D, Liang J, Yu H, Wu H, Xu C, Liu J*, Lai R*: A tick B-cell inhibitory protein from salivary glands of the hard tick, Hyalomma asiaticum asiaticum. Biochem Biophys Res Commun 2006, 343: 585-590.

[8]Xu X, Li J, Han Y, Yang H, Liang J, Lu Q, Lai R*: Two antimicrobial peptides from skin secretions of Rana grahami. Toxicon 2006, 47: 459-464.

[9]Zhang YQ, Li WJ, Zhang KY, Tian XP, Jiang Y, Xu LH, Jiang CL,Li WJ*,Lai R*: Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China. Int J Syst Evol Microbiol 2006, 56: 459-463.

[10]Xu X, Li J, Lu Q, Yang H, Zhang Y,Lai R*: Two families of antimicrobial peptides from wasp (Vespa magnifica) venom. Toxicon 2006, 47: 249-253.

[11]Li J, Xu X, Yu H, Yang H, Huang Z, Lai R*: Direct antimicrobial activities of PR-bombesin. Life Sci 2006, 78: 1953-1956.

[12]Yu D, Sheng Z, Xu X, Li J, Yang H, Liu Z, Rees HH, Lai R*: A novel antimicrobial peptide from salivary glands of the hard tick, Ixodes sinensis. Peptides 2006, 27: 31-35.

2005年发表论文

[1]Zhang YX, Lai R, Lee WH, Zhang Y: Frog albumin is expressed in skin and characterized as a novel potent trypsin inhibitor. Protein Sci 2005, 14: 2469-2477.

[2]Lee WH, Zhang J, Zhang YX, Jin Y, Lai R, Zhang Y*: Maximin 9, a novel free thiol containing antimicrobial peptide with antimycoplasma activity from frog Bombina maxima. FEBS Lett 2005 , 579: 4443-4448.

[3]Lee WH, Liu SB, Shen JH, Jin Y,Lai R,Zhang Y*: Identification and molecular cloning of a novel neuromedin U analog from the skin secretions of toad Bombina maxima. Regul Pept 2005, 129: 43-47.

[4]Lee WH, Li Y, Lai R, Li S, Zhang Y*, Wang W*: Variety of antimicrobial peptides in the Bombina maxima toad and evidence of their rapid diversification. Eur J Immunol 2005, 35: 1220-1229.

[5]Liang JG, Zhang J, Lai R, Rees HH: An opioid peptide from synganglia of the tick, Amblyomma testindinarium. Peptides 2005, 26: 603-606.

2004年以前发表论文

[1]Lai R#, Takeuchi H, Lomas LO, Jonczy J, Rigden DJ, Rees HH, Turner PC: A new type of antimicrobial protein with multiple histidines from the hard tick, Amblyomma hebraeum. FASEB J 2004, 18: 1447-1449.

[2]Lai R#, Takeuchi H, Jonczy J, Rees HH, Turner PC: A thrombin inhibitor from the ixodid tick, Amblyomma hebraeum. Gene 2004, 342: 243-249.

[3]Lai R#, Liu H, Lee WH, Zhang Y*: Bombinakinin M gene associated peptide, a novel bioactive peptide from skin secretions of the toad Bombina maxima. Peptides 2003, 24: 199-204.

[4]Lai R, Liu H, Lee WH, Zhang Y*: Two novel Bv8-like peptides from skin secretions of the toad Bombina maxima. Comp Biochem Physiol B 2003, 134: 509-514.

[5]Lai R, Yang DM, Lee WH, Zhang Y*: Biological activities of skin secretions of the salamander Tylototriton verrucosus. J Nat Toxins 2002, 11: 245-250.

[6]Lai R, Liu H, Hui Lee W, Zhang Y*: An anionic antimicrobial peptide from toad Bombina maxima. Biochem Biophys Res Commun 2002, 295: 796-799.

[7]Lai R, Liu H, Lee WH, Zhang Y*: A novel proline rich bombesin-related peptide (PR-bombesin) from toad Bombina maxima. Peptides 2002, 23: 437-442.

[8]Lai R, Zheng YT, Shen JH, Liu GJ, Liu H, Lee WH, Tang SZ, Zhang Y*: Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima. Peptides 2002, 23: 427-435.

[9]Lai R, Liu H, Lee WH, Zhang Y*: Identification and cloning of a trypsin inhibitor from skin secretions of Chinese red-belly toad Bombina maxima. Comp Biochem Physiol B 2002, 131: 47-53.

[10]Lai R, Liu H, Hui Lee W, Zhang Y*: A novel bradykinin-related peptide from skin secretions of toad Bombina maxima and its precursor containing six identical copies of the final product. Biochem Biophys Res Commun 2001, 286: 259-263.

申请专利

[1]大蹼铃蟾多肽在制备抗肿瘤和艾滋病药物中的应用。专利号：ZL 00122417.4

[2]大蹼铃蟾抑制剂及其制备方法和在制药中的应用。专利号：ZL 00122416.6

[3]大蹼铃蟾抗精子多肽及其制备方法和在制药中的应用。专利号：ZL 00130617.0

[4]大蹼铃蟾抗微生物多肽，制备方法和应用。专利号：ZL 00122415.8

[5]血管收缩因子及其制备方法和在制药中的应用。专利号：ZL 02113517.7

[6]酸性抗菌肽及其基因和在制药中的应用。专利号：ZL 02113515.0

[7]饱食因子及其制备方法和其基因。专利号：ZL 02113516.9

[8]大蹼铃蟾抗肿瘤蛋白及其制备方法和其基因。专利号：ZL 01107253.9

[9]大蹼铃蟾抗微生物肽及其制备方法和其基因。专利号：ZL 01107255.5

[10]大蹼铃蟾血管扩张因子及其制备方法和其基因。专利号：ZL01107254.7

[11]大蹼铃蟾神经营养因子及其制备方法和其基因。专利申请号：ZL01108520.7

[12]大蹼铃蟾神经营养肽及其制备方法和其基因。专利申请号：01108519.3

[13]大蹼铃蟾神经缓激肽及其制备方法和其基因。专利申请号：01108522.3

[14]大蹼铃蟾铃蟾肽及其制备方法和其基因。专利申请号：01108519.3

[15]大蹼铃蟾抗菌多肽，制备方法和应用。专利申请号：00122414.X

[16]胡蜂抗菌肽及其制备方法和应用。申请号：200510010664.7

[17]中华硬蜱神经肽及其制备方法和应用。专利号：ZL 200510010665.1

[18]棕点湍蛙抗菌肽及其基因和在制药中的应用。申请号：ZL200610010627.0

[19]棕点湍蛙活性肽及其基因和在制药中的应用。申请号：ZL 200610010628.5

[20]无指盘臭蛙胰岛素释放促进因子和在制药中的应用，200610010769.7

[21]无指盘臭蛙胰岛素释放促进肽和在制药中的应用，申请号：ZL 200610010770.X

[22]无指盘臭蛙抗菌肽及其应用。申请号：200610010928.3

[23]无指盘臭蛙丙精肽、基因和变异体及其在制药中的应用。申请号：200610010931.5

[24]无指盘臭蛙抗肿瘤多肽及其应用。申请号：200610010929.8

[25]无指盘臭蛙丝氨酸蛋白酶抑制剂及其基因和应用。申请号：200610010930.0

[26]棕点湍蛙胰岛素释放促进肽和在制药中的应用。申请号：200610010932.X

[27]一种新型环状小肽BA及其应用。申请号：200710065665.0

[28]无指盘臭蛙蛙皮肽及其基因和在制药中应用。申请号：200710065666.5

[29]无指盘臭蛙抑生肽、其基因和变异体及在制药中应用。申请号：200710065667.X

[30]无指盘臭蛙皮肤活性肽及其基因和在制药中应用。专利申请号：200710065668.4

[31]无指盘臭蛙免疫调节肽、其基因和变异体及其在制药中应用。专利申请号：200710065669.9

[32]无指盘臭蛙抗微生物多肽及其基因和在制药中应用。申请号：200710065670.1

[33]无指盘臭蛙分泌肽及其基因和在制药中应用。申请号：200710065672.0

[34]无指盘臭蛙臭蛙肽及其基因和在制药中应用。申请号：200710065674.X

[35]姚虻唾液腺免疫调节肽及其基因和应用。申请号：200810058075.X